Aims of the CIMR microscopy facility
The primary aim of the CIMR microscopy facility is to facilitate high-quality cell biological research by providing the very latest and best instrumentation and imaging software to researchers at the institute. We also aim to ensure that the facility is used to maximum effect by providing comprehensive training and on-going support on all instruments and technologies available. The variety of instruments and image analysis options in the facility ensure that we are able to meet most imaging requirements in-house.
We are constantly assessing the requirements of the institute and developing the facility accordingly; most recently this has resulted in the expansion of our confocal capacity, the addition of an upright microscope capable of imaging large areas and the addition of a super-resolution microscope incorporating two distinct technologies.
The facility is used by the majority of researchers and research groups at the CIMR and provides invaluable tools for investigating cellular processes in both fixed and living cells in 2, 3 or even 4 dimensions. This is achieved by means of conventional imaging, HCS, confocal microscopy, time-lapse, real-time widefield & TIRF, FRAP, FRET and FCS.
Our resources include:
- Widefield epi-flourescence and colour brightfield microscopes in upright and inverted configurations
- Fully incubated laser scanning confocal microscopes
- Fully incubated, combined Spinning Disk Confocal / Total Internal Reflection Fluorescence (TIRF) instrument
- High Content Screening and Analysis instrument (Thermo Arrayscan VTi)
- Super-resolution microscope offering both super-resolution structured illumination (SSIM) and localisation microscopy (e.g. PALM and STORM)
- Image analysis workstations with leading software packages (e.g. Volocity, Imaris, ZEN, FiJi)
- Histology processing, cutting and staining equipment
The facility is managed by two experienced microscopists, Matthew Gratian and Mark Bowen, who provide all training and support on the above instruments.