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Cambridge Institute for Medical Research

 

Unravelling the molecular pathology of axon death

General audience summary:
Hereditary spastic paraplegias (HSPs) are a subtype of motor neuron disease, and affected people develop progressive leg paralysis because some of the longest neuronal connections (‘axons’) degenerate. HSPs are caused by mutations in specific genes, most commonly in the gene that codes for the spastin protein. Our aim is to understand how spastin normally works and how this goes wrong in HSPs. We are currently focusing on a role of spastin in regulating the transport systems in a cell, specifically in controlled cutting of the transport tracks called ‘microtubules’. This detailed understanding should inform rational treatment approaches for hereditary spastic paraplegias, and perhaps other similar neurological conditions.

Strategic CIMR themes: Rare Genetic Diseases, Neurological Diseases, Membrane Trafficking, Organelle Biology

Funding: Medical Research Council, United States Spastic Paraplegia Foundation, Tom Wahlig Stiftung, NIHR Cambridge Biomedical Research Centre

Research Group members: Henry Cornish, Julia Kleniuk, Catherine Rodger, Eliska Zlamalova

Research

We focus on understanding cell biological mechanisms required for axonal health and thereby nervous system function by studying the molecular pathology of the hereditary spastic paraplegias (HSPs). These are human genetic conditions in which axons develop selective distal degeneration, so identifying the genes involved enables us to target with precision proteins that are vital in keeping axons alive.

We want to understand the normal functions of HSP proteins and how disruption of these functions causes axonal degeneration. Many of the disease proteins function in membrane traffic processes, especially at the endoplasmic reticulum and at endosomes. Our work concentrates on understanding the functions of this membrane traffic subgroup of HSP proteins.  Within this group, we primarily focus on the functions of spastin, a protein encoded by the gene most commonly mutated in HSP.

Through our work on spastin, we have discovered a cellular pathway that links groups of HSP proteins involved in endoplasmic reticulum shaping and endosomal tubule fission to a common feature of lysosome dysfunction. We propose that this is the key underlying pathology in most genetic subtypes of HSP.  We are now investigating the mechanisms by which lysosome dysfunction could cause axonal degeneration in HSP, with the aim of identifying tractable therapeutic targets.  In this work we are increasingly using human induced pluripotent stem cell-derived neurons, which we are genetically engineering to model different subtypes of HSP.  These are proving to be a powerful tool to model the disease and to test therapeutic strategies.

Publications

Key publications: 

Connell JW, Allison RJ, Rodger C, Pearson G, Zlamalova E, Reid E (2019) ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic. Cellular and Molecular Life Sciences, in press.

Shribman S, Reid E, Crosby AH, Houlden H, Warner TT (2019). Hereditary spastic paraplegia: from diagnosis to emerging therapeutic approaches. Lancet Neurology, S1474-4422(19)30235-2

Newton T, Allison R, Edgar JR, Lumb JH, Rodger CE, Manna PT, Rizo T, Kohl Z, Nygren AOH, Arning L, Schüle R, Depienne C, Goldberg L, Frahm C, Stevanin G, Durr A, Schöls L, Winner B, Beetz C & Reid E. Mechanistic basis of an epistatic interaction reducing age at onset in hereditary spastic paraplegia. Brain 141, 1286-1299 (2018).

Allison R, Edgar JR, Pearson G, Rizo T, Newton T, Günther S, Berner F, Hague J, Connell JW, Winkler J, Lippincot-Schwartz J, Beetz C, Winner B & Reid E. Defects in ER-endosome contacts impact lysosome function in hereditary spastic paraplegia. J. Cell Biol. 216, 1337-1355. PMCID: PMC5412567 (2017).

Highlighted as Science Editors’ choice: Science (2017) 356, 498. Recommended by Faculty of 1000. Selected for JCB “Special Collection of Outstanding Articles on Lysosomes and Endocytosis” (2018).

Allison R, Lumb JH, Fassier C, Connell JW, Ten Martin D, Seaman MNJ, Hazan J, Reid E. An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome. J. Cell Biol. 202, 527-543 PMCID:PMC3734076 (2013).

Montenegro G, Rebelo AP, Connell J, Allison R, Babalini C, D'Aloia M, Montieri P, Schüle R, Ishiura H, Price J, Strickland A, Gonzalez MA, Baumbach-Reardon L, Deconinck T, Huang J, Bernardi G, Vance JM, Rogers MT, Tsuji S, De Jonghe P, Pericak-Vance MA, Schöls L, Orlacchio A, Reid E & Züchner S. Mutations in the ER-shaping protein reticulon 2 cause the axon-degenerative disorder hereditary spastic paraplegia type 12. J. Clin. Invest. 122, 538-544 PMCID: PMC3266795 (2012).

Tsang HTH, Edwards TL, Wang X, Connell JW, Davies RJ, Durrington HJ, O’Kane CJ, Luzio JP & Reid E. The hereditary spastic paraplegia proteins NIPA1, spastin and spartin are inhibitors of mammalian BMP signaling. Hum. Mol. Genet. 18, 3805-3821 (2009).

Connell JW, Lindon C, Luzio JP & Reid E. Spastin couples microtubule severing to membrane traffic in completion of cytokinesis and secretion. Traffic 10, 42-56 PMCID:PMC2709849 (2009).

Wang X, Shaw RW, Tsang HT, Reid E, O’Kane CJ. Spichthyin, the Drosophila homolog of ichthyin and SPG6, is an inhibitor of BMP signaling, and can impair axonal transport.  Nature Neurosci. 10, 177-185 (2007).

Sanderson CM, Connell JW, Edwards TL, Bright NA, Duley S, Thompson A, Luzio JP & Reid E. Spastin and atlastin, two proteins mutated in autosomal-dominant hereditary spastic paraplegia, are binding partners. Hum. Mol. Genet. 15, 307-318 (2006).

Reid E, Connell J, Edwards TL, Duley S, Brown SE & Sanderson CM. The hereditary spastic paraplegia protein spastin interacts with the ESCRT-III complex-associated endosomal protein CHMP1B. Hum. Mol. Genet. 14, 19-38 (2005).

Reid E, Kloos M, Ashley-Koch A, Hughes L, Bevan S, Svenson IK, Lennon Graham F, Gaskell PC, Dearlove A, Pericak-Vance M, Rubinsztein DC & Marchuk DA. A kinesin heavy chain (KIF5A) mutation in hereditary spastic paraplegia (SPG10).  Am. J. Hum. Genet. 71, 1189-1194 (2002).

Professor of Neurogenetics and Molecular Neurobiology
Honorary Consultant, Medical Genetics

Contact Details

Takes PhD students
Available for consultancy

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College: 
St Edmund's College