The Flow Cytometry Core Facility is headed by Dr Reiner Schulte and provides state-of-the-art flow cytometry service which consists of expert advice for experimental design, individual instrument training, and data analysis. The facility is capable of performing cell sorting for researchers to isolate cell populations needed for further studies.
Cell Sorters
High speed Influx Cell Sorters (BD Biosciences).
One Influx cell sorter has five lasers at 355nm, 405nm, 488nm, 561nm, and 640nm and is equipped with 14 fluorescence detectors and a small particle detector.
The second Influx cell sorter has four lasers at 405nm, 488nm, 561nm, and 640nm and is equipped with 16 fluorescence detectors and a small particle detector.
The third Influx cell sorter has four lasers at 405nm, 488nm, 561nm, and 640nm and is equipped with 12 fluorescence detectors. This cell sorter is housed in a biosafety cabinet to allow sorting of clinical samples.
All Influx cell sorters can be used with different nozzle sizes (70µm, 86µm, 100µm or 130µm) and are able to separate up to six cell populations simultaneously.
Melody cell sorter (BD Bioscience)
The facility operates a three (405nm, 488nm, 561nm) laser BD Melody sorter equipped with 8 fluorescence detectors. This instrument is housed in a biosafety cabinet to allow sorting of clinical samples.
The Melody cell sorters operates with a 100µm nozzle and can separate up to four cell populations simultaneously.
Flow Cytometry Analysers
LSRFortessas (BD Bioscience).
Two LSRFortessas (355nm, 405nm, 488nm, 561nm, and 640nm) are able to detect 18 colours and both have the option to operate a high throughput sampler.
The third LSRFortessa (405nm, 488nm, 561nm, and 640nm) is able to detect 16 colours.
LSR II (BD Biosciences). The LSRII (355nm, 488nm, and 640nm) is housed at the Hutchison/MRC Research Centre and allows users to see two UV, five blue and two red fluorescent parameters. A high throughput sampler can be used on this instrument.
Attune NxT (Thermofisher). The Attune NxT (405nm, 488nm, and 561nm) uses acoustic-assisted hydrodynamic focusing and is able to detect 11 colours. A high throughput autosampler is attached to this instrument.
Accuri C6 (BD Biosciences). The C6 is equipped with a blue (488nm) and a red (640nm) laser and can measure four fluorescence parameters.
FACS Caliburs (BD Biosciences). One FACS Calibur flow cytometer with 488nm and 635nm lasers allow four colour analyses. A high throughput sampler can be used on this instrument.
